Transglutaminase 2 Antikörper

Produktnummer ABIN6940725

Produktdetails anti-Transglutaminase 2 Antikörper

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reaktivität: Human, Maus, Ratte, Affe, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transglutaminase 2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Spezifität: Recognizes a 77-85 kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human TGM2 protein

Klon: SPM358

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HUVEC cells. Endothelial cells in placenta, liver, brain or breast carcinoma. Smooth muscle cells of any origin (e. G. Intestine).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL, 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Andere Bezeichnung: TGM2 (TGM2 Produkte)

Synonyme: G-ALPHA-h antikoerper, GNAH antikoerper, TG2 antikoerper, TGC antikoerper, tgm2 antikoerper, zgc:101081 antikoerper, TgaseII antikoerper, TGM2 antikoerper, fc12b04 antikoerper, wu:fc12b04 antikoerper, zgc:136700 antikoerper, TGL2 antikoerper, G[a]h antikoerper, TGase2 antikoerper, tTG antikoerper, tTGas antikoerper, transglutaminase 2 antikoerper, transglutaminase 2, C polypeptide A antikoerper, transglutaminase 2b antikoerper, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) antikoerper, transglutaminase 2, C polypeptide antikoerper, TGM2 antikoerper, tgm2a antikoerper, Tgm2 antikoerper, tgm2b antikoerper

Molekulargewicht: 77-85kDa

Gen-ID: 7052

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling