Vinculin Antikörper (AA 174-322)

Produktnummer ABIN6940866

Produktdetails anti-Vinculin Antikörper

Target: Vinculin (VCL)

Bindungsspezifität: AA 174-322

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vinculin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary)

Klon: VCL-2575

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431, K562, NIH3T3, U-87, THP-1 cells. Human Bladder and Testis.

Known Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Vinculin

Target: Vinculin (VCL)

Andere Bezeichnung: VCL (VCL Produkte)

Hintergrund: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin. In addition, vinculin, Talin and -Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Molekulargewicht: 117kDa

Gen-ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2