Villin 1 Antikörper (AA 179-311)

Produktnummer ABIN6940871

Produktdetails anti-Villin 1 Antikörper

Target: Villin 1 (VIL1)

Bindungsspezifität: AA 179-311

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Villin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Spezifität: Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary)

Klon: VIL1-1314

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.

Known Application: Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Villin 1

Target: Villin 1 (VIL1)

Andere Bezeichnung: VIL1 (VIL1 Produkte)

Synonyme: D2S1471 antikoerper, VIL antikoerper, Vil antikoerper, vil1 antikoerper, MGC64339 antikoerper, VIL1 antikoerper, vil1l antikoerper, zgc:55779 antikoerper, villin 1 antikoerper, villin 1 S homeolog antikoerper, VIL1 antikoerper, Vil1 antikoerper, vil1.S antikoerper, vil1 antikoerper

Molekulargewicht: 93kDa

Gen-ID: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization