Cyclin B1 Antikörper
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- Target Alle Cyclin B1 (CCNB1) Antikörper anzeigen
- Cyclin B1 (CCNB1)
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Reaktivität
- Human, Maus
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Cyclin B1 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Staining Methods (StM)
- Spezifität
- It recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
- Aufreinigung
- Purified by Protein A/G
- Immunogen
- Recombinant human full-length CCNB1 protein
- Klon
- CCNB1-1098
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CCNB1 Primärantikörper
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- Applikationshinweise
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Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Haltbarkeit
- 24 months
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- Target
- Cyclin B1 (CCNB1)
- Andere Bezeichnung
- CCNB1 (CCNB1 Produkte)
- Synonyme
- ccnb antikoerper, cycb antikoerper, cb267 antikoerper, cycb1 antikoerper, wu:fa19g04 antikoerper, wu:fb16d01 antikoerper, wu:fb16e07 antikoerper, wu:fi21c01 antikoerper, ccnb1 antikoerper, MGC53596 antikoerper, CCNB antikoerper, Ccnb1-rs1 antikoerper, Ccnb1-rs13 antikoerper, CycB1 antikoerper, Cycb-4 antikoerper, Cycb-5 antikoerper, Cycb1-rs1 antikoerper, cyclin B1 antikoerper, cyclin B1 S homeolog antikoerper, ccnb1 antikoerper, ccnb1.2 antikoerper, ccnb1.S antikoerper, CCNB1 antikoerper, Ccnb1 antikoerper, ccnb1.2.S antikoerper
- Molekulargewicht
- 55-62kDa
- Gen-ID
- 891
- UniProt
- P14635
- Pathways
- Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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