CD3 epsilon Antikörper (Cytoplasmic Domain)

Produktnummer ABIN6941021

Dieses Anti-CD3 epsilon-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD3 epsilon in FACS, WB und IF. Geeignet für Human, Ratte, Schwein, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen und Huhn.

Kurzübersicht für CD3 epsilon Antikörper (Cytoplasmic Domain) (ABIN6941021)

Target: CD3 epsilon (CD3E)

Reaktivität: Human, Ratte, Schwein, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Klon: PC3-188A

Produktdetails anti-CD3 epsilon Antikörper

Bindungsspezifität: AA 156-168, Cytoplasmic Domain

Spezifität: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Aufreinigung: Purified by Protein A/G

Immunogen: A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3-ε chain

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat cells. Tonsil and lymph node.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL for 60 minutes at RT), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E

Molekulargewicht: 20kDa

Gen-ID: 916

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway