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CD3 epsilon Antikörper (AA 23-119)

Name: CD3 epsilon Antikörper (AA 23-119)
Brand: antibodies-online
SKU: ABIN6941022
Price: 663.89 EUR
Availability: InStock

CD3E Reaktivität: Human FACS, WB, IHC, ELISA, Coat, StM Wirt: Maus Monoclonal C3e-1931 unconjugated

Western Blot Analysis of human Jurkat cell lysate using CD3e Mouse Monoclonal Antibody (C3e/1931). (CD3 epsilon Antikörper (AA 23-119))

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD3e-Monospecific Mouse Monoclonal Antibody (C3e/1931) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD3 epsilon Antikörper (AA 23-119))

PAr

Flow Cytometric Analysis of Jurkat cells. (CD3 epsilon Antikörper (AA 23-119))

FACS

Formalin-fixed, paraffin-embedded human Spleen stained with CD3e Mouse Monoclonal Antibody (C3e/1931). (CD3 epsilon Antikörper (AA 23-119))

IHC

SDS-PAGE Analysis Purified CD3e Mouse Monoclonal Antibody (C3e/1931). (CD3 epsilon Antikörper (AA 23-119))

SDS

5 Abbildungen

Produktnummer ABIN6941022

Datenblatt (PDF)

Target Alle CD3 epsilon (CD3E) Antikörper anzeigen

CD3 epsilon (CD3E)
Bindungsspezifität
24

19

16

16

12

10

9

8

6

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 23-119
Reaktivität
228

176

51

42

18

8

8

8

7

4

4

4

4

3

3

3

3

3

3

1

1

1

1

1
Human
Wirt
138

123

46

23

23

10

1

1

1
Maus
Passende Sekundärantikörper
Klonalität
248

112

6
Monoklonal
Konjugat
141

33

29

21

18

8

8

6

5

5

5

4

4

4

4

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Dieser CD3 epsilon Antikörper ist unkonjugiert
Applikation
268

146

104

86

84

66

31

27

25

22

14

13

13

13

7

6

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Spezifität

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Aufreinigung

Purified by Protein A/G

Immunogen

A recombinant human CD3e protein fragment (around aa 23-119) (exact sequence is proprietary)

Klon

C3e-1931

Isotyp

IgG1 kappa

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CD3E Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-CD3 epsilon (CD3E) (AA 23-207) antibody
CD3E Reaktivität: Human WB, IHC, IF, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-CD3 epsilon (CD3E) antibody
CD3E Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Chimeric YTH 12-5 unconjugated Recombinant Antibody

Applikationshinweise

Positive Control: Jurkat cells. Tonsil or lymph node.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit

24 months
Target

CD3 epsilon (CD3E)

Andere Bezeichnung

CD3E (CD3E Produkte)

Molekulargewicht

20kDa

Gen-ID

916

UniProt

P07766

Pathways

T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway