CD3 epsilon Antikörper

Produktnummer ABIN6941023

Produktdetails anti-CD3 epsilon Antikörper

Target: CD3 epsilon (CD3E)

Reaktivität: Human, Schimpanse

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat), Immunohistochemistry (Acetone-fixed) (IHC (af))

Spezifität: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Aufreinigung: Purified by Protein A/G

Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor

Klon: UCHT1

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human peripheral blood. Jurkat cells. Tonsil or lymph node.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL). ,Immunohistochemistry (Acetone-fixed frozen tissues) (1-2 μg/mL for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E (CD3E Produkte)

Molekulargewicht: 20kDa

Gen-ID: 916

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway