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CD14 Antikörper (AA 25-148)

Name: CD14 Antikörper (AA 25-148)
Brand: antibodies-online
SKU: ABIN6941093
Price: 640.2 EUR
Availability: InStock

CD14 Reaktivität: Human FACS, WB, IHC, ISt, StM Wirt: Maus Monoclonal LPSR-2385 unconjugated

Flow Cytometric Analysis of A549 cells using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 Antikörper (AA 25-148))

FACS

Western Blot Analysis of A549 cell lysate using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 Antikörper (AA 25-148))

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD14 Antikörper (AA 25-148))

PAr

Formalin-fixed, paraffin-embedded human Lymph Node stained with CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 Antikörper (AA 25-148))

IHC

Formalin-fixed, paraffin-embedded human Tonsil stained with CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 Antikörper (AA 25-148))

IHC

SDS-PAGE Analysis Purified CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 Antikörper (AA 25-148))

SDS

6 Abbildungen

Produktnummer ABIN6941093

Datenblatt (PDF)

Target Alle CD14 Antikörper anzeigen

CD14
Bindungsspezifität
8

6

5

5

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 25-148
Reaktivität
244

45

32

25

24

18

17

16

15

14

13

10

9

9

8

8

8

5

2

2

2

1

1

1
Human
Wirt
213

52

25

3

1
Maus
Passende Sekundärantikörper
Klonalität
240

54
Monoklonal
Konjugat
115

31

24

18

15

8

6

5

5

5

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD14 Antikörper ist unkonjugiert
Applikation
219

82

59

56

52

49

21

14

13

12

6

6

4

4

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Spezifität

Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Aufreinigung

Purified by Protein A/G

Immunogen

Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)

Klon

LPSR-2385

Isotyp

IgG1 kappa

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Applikationshinweise

Positive Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.
Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit

24 months
Target

CD14

Andere Bezeichnung

CD14 (CD14 Produkte)

Synonyme

CD14 molecule antikoerper, CD14 antigen antikoerper, CD14 antikoerper, Cd14 antikoerper

Molekulargewicht

55kDa

Gen-ID

929

UniProt

P08571

Pathways

TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades