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CD27 Antikörper

Name: CD27 Antikörper
Brand: antibodies-online
SKU: ABIN6941124
Price: 640.2 EUR
Availability: InStock

CD27 Reaktivität: Human FACS, ELISA, IHC, IF, StM, Coat Wirt: Maus Monoclonal LPFS2-1611 unconjugated

Flow Cytometric Analysis of Ramos cells using CD27 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (CD27 Antikörper)

FACS

SDS-PAGE Analysis Purified CD22 Mouse Monoclonal Antibody (LPFS2/1611). (CD27 Antikörper)

SDS

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD27 Mouse Monoclonal Antibody (LPFS2/1611) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD27 Antikörper)

PAr

Formalin-fixed, paraffin-embedded human Colon stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611). (CD27 Antikörper)

IHC

Formalin-fixed, paraffin-embedded human Spleen stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611). (CD27 Antikörper)

IHC

Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611). (CD27 Antikörper)

IHC

Immunofluorescence staining of Ramos cells using CD27 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). (CD27 Antikörper)

8 Abbildungen

Produktnummer ABIN6941124

Datenblatt (PDF)

Target Alle CD27 Antikörper anzeigen

CD27 (CD27 Molecule (CD27))
Reaktivität
195

71

52

5

5

5

3

2

1

1

1
Human
Wirt
110

86

22

11

3

1

1
Maus
Passende Sekundärantikörper
Klonalität
137

95

2
Monoklonal
Konjugat
76

24

23

15

13

7

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
Dieser CD27 Antikörper ist unkonjugiert
Applikation
151

81

50

38

32

20

17

14

13

13

11

9

6

4

4

3

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM), Coating (Coat)

Spezifität

Recognizes a protein of a disulfide-linked 120 kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.

Aufreinigung

Purified by Protein A/G

Immunogen

Recombinant full-length human CD27 protein

Klon

LPFS2-1611

Isotyp

IgG1 kappa

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anti-CD27 Molecule (CD27) (AA 229-312) antibody
CD27 Reaktivität: Human ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-CD27 Molecule (CD27) (AA 196-225), (C-Term) antibody
CD27 Reaktivität: Human FACS, WB Wirt: Kaninchen Polyclonal RB33726 unconjugated

anti-CD27 Molecule (CD27) antibody
CD27 Reaktivität: Human FACS, IHC, StM Wirt: Maus Monoclonal rLPFS2-1611 unconjugated Recombinant Antibody

anti-CD27 Molecule (CD27) antibody
CD27 Reaktivität: Human WB, IF Wirt: Kaninchen Monoclonal unconjugated

Applikationshinweise

Positive Control: Ramos cells. Human peripheral blood lymphocytes. Ramos and lymph node cell lysates. Human tonsil and stomach tissues.
Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit

24 months
Target

CD27 (CD27 Molecule (CD27))

Andere Bezeichnung

CD27 (CD27 Produkte)

Synonyme

TNFRSF7 antikoerper, S152 antikoerper, Tnfrsf7 antikoerper, Tp55 antikoerper, S152. LPFS2 antikoerper, T14 antikoerper, CD27 molecule antikoerper, CD27 antigen antikoerper, CD27 antikoerper, Cd27 antikoerper

Molekulargewicht

120kDa

Gen-ID

939

UniProt

P26842

Pathways

Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints