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CD47 Antikörper (AA 18-135)

Name: CD47 Antikörper (AA 18-135)
Brand: antibodies-online
SKU: ABIN6941215
Price: 640.2 EUR
Availability: InStock

CD47 Reaktivität: Human FACS, IF, IHC, ELISA, StM, Coat Wirt: Maus Monoclonal CD47-3019 unconjugated

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD47 Mouse Monoclonal Antibody (IAP/3019). (CD47 Antikörper (AA 18-135))

IHC

SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody (IAP/3019). (CD47 Antikörper (AA 18-135))

SDS

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD47 Mouse Monoclonal Antibody (CD47/3019) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD47 Antikörper (AA 18-135))

PAr

Flow Cytometric Analysis of MCF-7 cells using CD47 Mouse Monoclonal Antibody (IAP/3019) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red). (CD47 Antikörper (AA 18-135))

FACS

IHC

Immunofluorescence staining of MCF-7 cells using CD47 Mouse Monoclonal Antibody (IAP/3019) followed by goat anti-Mouse IgG conjugated to CF488 (green). (CD47 Antikörper (AA 18-135))

6 Abbildungen

Produktnummer ABIN6941215

Datenblatt (PDF)

Target Alle CD47 Antikörper anzeigen

CD47
Bindungsspezifität
16

15

8

8

7

4

4

2

2

2

2

2

1

1

1

1

1

1

1

1
AA 18-135
Reaktivität
121

52

27

6

3

2

2

2

1

1

1

1
Human
Wirt
78

64

9

1
Maus
Passende Sekundärantikörper
Klonalität
77

73

1
Monoklonal
Konjugat
66

13

12

7

5

5

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
Dieser CD47 Antikörper ist unkonjugiert
Applikation
87

85

41

39

34

26

26

18

16

16

14

10

7

4

3

3

3

3

2

2

2

2

2

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Staining Methods (StM), Coating (Coat)

Spezifität

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Aufreinigung

Purified by Protein A/G

Immunogen

A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)

Klon

CD47-3019

Isotyp

IgG1 kappa

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CD47 Reaktivität: Human, Maus, Ratte WB, IF, IHC, ELISA, ICC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Positive Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC).
Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit

24 months
Target

CD47

Andere Bezeichnung

CD47 (CD47 Produkte)

Synonyme

IAP antikoerper, MER6 antikoerper, OA3 antikoerper, 9130415E20Rik antikoerper, AA407862 antikoerper, AI848868 antikoerper, AW108519 antikoerper, B430305P08Rik antikoerper, Itgp antikoerper, CD47/IAP antikoerper, alkaline phosphatase, intestinal antikoerper, CD47 molecule antikoerper, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antikoerper, Cd47 molecule antikoerper, ALPI antikoerper, CD47 antikoerper, Cd47 antikoerper

Molekulargewicht

~50kDa

Gen-ID

961

UniProt

Q08722