CD72 Antikörper

Produktnummer ABIN6941252

Produktdetails anti-CD72 Antikörper

Target: CD72 (CD72 Molecule (CD72))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD72 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Aufreinigung: Purified by Protein A/G

Immunogen: HF B1 plasmacytoid cell line.

Klon: BU40

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: K562 cel lysate. Tonsil or Lymph Node.

Known Application: ELISA (For coating, order without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD72

Target: CD72 (CD72 Molecule (CD72))

Andere Bezeichnung: CD72 (CD72 Produkte)

Synonyme: CD72c antikoerper, Ly-19 antikoerper, Ly-32 antikoerper, Ly-m19 antikoerper, Lyb-2 antikoerper, CD72b antikoerper, LYB2 antikoerper, CD72 antigen antikoerper, CD72 molecule antikoerper, Cd72 antikoerper, CD72 antikoerper

Hintergrund: CD5 has been identified as a transmembrane glycoprotein that is expressed on 70 % of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/CD3 chain, and with the Src family kinase, Lck p56. The C-type lectin superfamily member CD72 is a cell surface negative regulator of B cell activation from the pro-B through the mature B cell stage. CD72 serves as a receptor for CD5. The ability of lymphocytes to respond to antigenic or mitogenic stimulation utilizes both positive and negative regulatory proteins that influence the threshold for responsiveness. The human CD72 gene maps to chromosome 9p13.3 and encodes a transmembrane glycoprotein that contains an ITIM. Upon tyrosine phosphorylation, the CD72 ITIM recruits SH2-containing phosphatases such as SHP-1, resulting in downregulation of cell activation. CD72-/- mice contain hyperproliferative B cells.

Molekulargewicht: 45kDa

Gen-ID: 971

UniProt: P21854

Pathways: BCR Signaling