Rekombinanter CD74 Antikörper

Produktnummer ABIN6941259

Produktdetails anti-CD74 Antikörper

Target: CD74 (HLA-DR-gamma (CD74))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Pavian

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD74 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Spezifität: It recognizes a protein of ~35 kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Keine Kreuzreaktivität: Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: SU-DHL-4 lymphoma cells

Klon: CLIP-3127R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: 293T, Daudi or Raji cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD74

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74 (CD74 Produkte)

Synonyme: DHLAG antikoerper, HLADG antikoerper, II antikoerper, Ia-GAMMA antikoerper, CLIP antikoerper, Ii antikoerper, INVG34 antikoerper, Iclp-1 antikoerper, iclp1 antikoerper, wu:fb11f09 antikoerper, wu:fb12a01 antikoerper, wu:fb13d06 antikoerper, wu:fk94e07 antikoerper, Iclp-2 antikoerper, cd74 antikoerper, iclp2 antikoerper, zgc:123161 antikoerper, CD74 molecule antikoerper, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antikoerper, CD74 molecule, major histocompatibility complex, class II invariant chain a antikoerper, CD74 molecule, major histocompatibility complex, class II invariant chain b antikoerper, CD74 antikoerper, Cd74 antikoerper, cd74a antikoerper, cd74b antikoerper

Molekulargewicht: 33-41kDa

Gen-ID: 972

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints