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Keratin 5/8 Antikörper

KRT5/8 Reaktivität: Human, Maus, Schwein, Ratte, Hund, Hamster, Rind (Kuh), Schaf, Frettchen WB, FACS, IF, IHC, StM Wirt: Maus Monoclonal C-50 unconjugated
Produktnummer ABIN6941336
  • Target Alle Keratin 5/8 (KRT5/8) Produkte
    Keratin 5/8 (KRT5/8)
    Reaktivität
    • 22
    • 15
    • 14
    • 12
    • 12
    • 12
    • 11
    • 9
    • 8
    • 3
    • 2
    • 2
    Human, Maus, Schwein, Ratte, Hund, Hamster, Rind (Kuh), Schaf, Frettchen
    Wirt
    • 22
    Maus
    Klonalität
    • 22
    Monoklonal
    Konjugat
    • 9
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Keratin 5/8 Antikörper ist unkonjugiert
    Applikation
    • 16
    • 15
    • 12
    • 12
    • 8
    • 8
    • 6
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
    Spezifität
    It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
    Keine Kreuzreaktivität
    Huhn, Xenopus laevis
    Aufreinigung
    Purified by Protein A/G
    Immunogen
    Cytoskeletal preparation from HeLa cells
    Klon
    C-50
    Isotyp
    IgG1 kappa
  • Applikationshinweise

    Positive Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    200 μg/mL
    Buffer
    0.5 mL at 200 µg/mL of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10 mM PBS, pH 7.4 with 0.05 % BSA and 0.05 % sodium azide. Contact us if you require this antibody in a different format.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2 to 8°C. Antibody is stable for 24 months.
    Haltbarkeit
    24 months
  • Target
    Keratin 5/8 (KRT5/8)
    Andere Bezeichnung
    KRT5 & KRT8 (KRT5/8 Produkte)
    Molekulargewicht
    58kDa (CK5), 52.5kDa (CK8)
    Gen-ID
    3852, 3856
    UniProt
    P13647, P05787
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