Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

Biotin Antikörper

Name: Biotin Antikörper
Brand: antibodies-online
SKU: ABIN6941338
Price: 640.2 EUR
Availability: InStock

WB, FACS, IHC, IF, ISt, CM Wirt: Maus Monoclonal Hyb-8 unconjugated

Formalin-fixed, paraffin-embedded human Tonsil stained with Biotinylated Lambda Light Chain probe followed by Anti-Biotin Mouse Monoclonal Antibody (Hyb-8). (Biotin Antikörper)

IHC

SDS-PAGE Analysis of Purified anti-Biotin Mouse Monoclonal antibody (Hyb-8). (Biotin Antikörper)

SDS

2 Abbildungen

Produktnummer ABIN6941338

Datenblatt (PDF)

Target Alle Biotin Produkte

Biotin

Reaktivität

Bitte anfragen
Wirt
56

34

28
Maus
Passende Sekundärantikörper
Klonalität
75

36
Monoklonal
Konjugat
29

11

8

8

7

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Biotin Antikörper ist unkonjugiert
Applikation
54

49

43

35

32

19

14

12

12

12

9

9

5

4

4

3

2

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Confocal Microscopy (CM)

Spezifität

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Aufreinigung

Purified by Protein A/G

Immunogen

Biotinylated sheep immunoglobulin

Klon

Hyb-8

Isotyp

IgG1 kappa
anti-Biotin antibody
WB, ELISA, IP, DB Wirt: Kaninchen Polyclonal unconjugated

anti-Biotin antibody
ELISA Wirt: Ziege Polyclonal unconjugated Fab fragment

anti-Biotin antibody
IHC, ISt Wirt: Kaninchen Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody (DyLight 488)
WB, FM, FLISA Wirt: Ziege Polyclonal DyLight 488

anti-Biotin antibody
FACS, ELISA Wirt: Maus Monoclonal SB58c unconjugated

anti-Biotin antibody
FACS, ELISA, IP, CyTOF Wirt: Maus Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
IP, Con Wirt: Maus Monoclonal unconjugated

anti-Biotin antibody
WB, FACS, ELISA, IHC, IP, Lbl Wirt: Ziege unconjugated

anti-Biotin antibody
IP, Con Wirt: Kaninchen Polyclonal unconjugated

anti-Biotin antibody
Reaktivität: Human WB, ELISA, IHC Wirt: Maus Monoclonal B-E24 unconjugated

Applikationshinweise

Positive Control: Biotinylated proteins in solution or on tissues.
Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit

24 months
Target

Biotin

Andere Bezeichnung

Biotin (Vitamin B7 or Vitamin H) (Biotin Produkte)

Substanzklasse

Chemical

Molekulargewicht

244Da