Keratin 7/17 Antikörper

Produktnummer ABIN6941343

Produktdetails anti-Keratin 7/17 Antikörper

Target: Keratin 7/17 (KRT7/17)

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 7/17 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Spezifität: It recognizes intermediate filament proteins (IFP's) of 55 kDa and 46 kDa, which are identified as cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation.

Keine Kreuzreaktivität: Frettchen, Maus, Kaninchen, Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: Cytoskeletal preparation from HeLa cells (human cervical carcinoma cells)

Klon: C-46

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa or ME-180 cells. Skin or hepatocellular carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Keratin 7/17

Target: Keratin 7/17 (KRT7/17)

Andere Bezeichnung: KRT7 & KRT17 (KRT7/17 Produkte)

Synonyme: KRT7 antikoerper, CK7 antikoerper, keratin 7 antikoerper, KRT7 antikoerper

Molekulargewicht: 55kDa (CK7), 46kDa (CK17)

Gen-ID: 3855, 3872

UniProt: P08729, Q04695