Macrophage / Histiocytoma Marker Antikörper

Produktnummer ABIN6941389

Produktdetails anti-Macrophage / Histiocytoma Marker Antikörper

Target: Macrophage / Histiocytoma Marker

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Macrophage / Histiocytoma Marker Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Spezifität: In Western blotting, it detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Keine Kreuzreaktivität: Maus, Schwein, Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: Membrane preparation from human hepatocytes

Klon: D11

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Liver or Histiocytoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Macrophage / Histiocytoma Marker

Target: Macrophage / Histiocytoma Marker

Andere Bezeichnung: Macrophage & Histiocytoma Marker (Macrophage / Histiocytoma Marker Produkte)

Molekulargewicht: 125kDa -135kDa