Keratin 77 Antikörper

Produktnummer ABIN6941429

Produktdetails anti-Keratin 77 Antikörper

Target: Keratin 77 (KRT77)

Reaktivität: Human, Ratte, Maus, Kaninchen, Rind (Kuh), Hund, Huhn, Affe, Schildkröte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 77 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Spezifität: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Aufreinigung: Purified by Protein A/G

Immunogen: Human epidermal keratin

Klon: AE-1

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Skin or squamous cell carcinoma (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Keratin 77

Target: Keratin 77 (KRT77)

Andere Bezeichnung: KRT77 (KRT77 Produkte)

Synonyme: K1B antikoerper, KRT1B antikoerper, 4732484G22Rik antikoerper, K1emb antikoerper, Krt239 antikoerper, mK1b antikoerper, Kb39 antikoerper, keratin 77 antikoerper, KRT77 antikoerper, Krt77 antikoerper

Molekulargewicht: 40-56.5kDa

Gen-ID: 374454

UniProt: Q7Z794