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Keratin 77 Antikörper

KRT77 Reaktivität: Human, Ratte, Maus, Kaninchen, Rind (Kuh), Hund, Huhn, Affe, Schildkröte IHC, WB, IF, FACS, StM Wirt: Maus Monoclonal AE-1 unconjugated
Produktnummer ABIN6941429
  • Target Alle Keratin 77 (KRT77) Antikörper anzeigen
    Keratin 77 (KRT77)
    Reaktivität
    • 25
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Ratte, Maus, Kaninchen, Rind (Kuh), Hund, Huhn, Affe, Schildkröte
    Wirt
    • 18
    • 7
    Maus
    Klonalität
    • 16
    • 9
    Monoklonal
    Konjugat
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Keratin 77 Antikörper ist unkonjugiert
    Applikation
    • 10
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    Spezifität
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
    Aufreinigung
    Purified by Protein A/G
    Immunogen
    Human epidermal keratin
    Klon
    AE-1
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product KRT77 Primärantikörper
  • Applikationshinweise

    Positive Control: Skin or squamous cell carcinoma (IHC).

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Haltbarkeit
    24 months
  • Target
    Keratin 77 (KRT77)
    Andere Bezeichnung
    KRT77 (KRT77 Produkte)
    Synonyme
    K1B antikoerper, KRT1B antikoerper, 4732484G22Rik antikoerper, K1emb antikoerper, Krt239 antikoerper, mK1b antikoerper, Kb39 antikoerper, keratin 77 antikoerper, KRT77 antikoerper, Krt77 antikoerper
    Molekulargewicht
    40-56.5kDa
    Gen-ID
    374454
    UniProt
    Q7Z794
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