Melanoma Marker (MART-1 + Tyrosinase + Gp100) Antikörper
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- Target
- Melanoma Marker (MART-1 + Tyrosinase + Gp100)
- Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Unkonjugiert
- Applikation
- Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
- Spezifität
- This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
- Aufreinigung
- Purified by Protein A/G
- Immunogen
- Recombinant hMART-1 protein (DT101 & BC199), Extract of pigmented melanoma metastases from lymph nodes (HMB45), Recombinant tyrosinase protein (T311)
- Klon
- DT101-BC199-T311-HMB45
- Isotyp
- IgG2a kappa
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anti-Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody
Reaktivität: Human IHC, ISt, StM Wirt: Maus Monoclonal M2-7C10-M2-9E3-T311-HMB45 unconjugated
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- Applikationshinweise
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Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Haltbarkeit
- 24 months
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- Target
- Melanoma Marker (MART-1 + Tyrosinase + Gp100)
- Molekulargewicht
- 20-22kDa (doublet) (MART-1): 90-100kDa (gp100), 70-80kDa (Tyrosinase)
- Gen-ID
- 2315, 6490
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