PUM1 Antikörper

Produktnummer ABIN6944728

Produktdetails anti-PUM1 Antikörper

Target: PUM1 (Pumilio Homolog 1 (PUM1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PUM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant protein within human Pumilio 1 aa 1-200.

Klon: 7B2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PUM1

Target: PUM1 (Pumilio Homolog 1 (PUM1))

Andere Bezeichnung: PUM1 (PUM1 Produkte)

Synonyme: fb96a03 antikoerper, pumilio1 antikoerper, si:ch211-285e16.1 antikoerper, wu:fb96a03 antikoerper, wu:fb96a08 antikoerper, zgc:63671 antikoerper, PUM1 antikoerper, DKFZp459K0627 antikoerper, AA517475 antikoerper, Pumm antikoerper, mKIAA0099 antikoerper, HSPUM antikoerper, PUMH antikoerper, PUMH1 antikoerper, PUML1 antikoerper, pumilio RNA-binding family member 1 antikoerper, pumilio RNA binding family member 1 antikoerper, pum1 antikoerper, PUM1 antikoerper, Pum1 antikoerper

Hintergrund:

Synonyms: Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, PUMH, HSPUM, PUMH1, PUML1, SCA47

Background: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery. Plays a role in cytoplasmic sensing of viral infection. In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation.

Gen-ID: 9698

UniProt: Q14671