Topoisomerase I Antikörper

Produktnummer ABIN6945193

Produktdetails anti-Topoisomerase I Antikörper

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified by Protein A.

Immunogen: Human Topoisomerase I between 1-100 amino acids

Klon: 1D1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: Topoisomerase I (TOP1 Produkte)

Synonyme: CG6146 antikoerper, Dmel\\CG6146 antikoerper, TOP1 antikoerper, TopI antikoerper, Topo I antikoerper, TopoI antikoerper, dTopoI antikoerper, l(1)G0134 antikoerper, l(1)G0201 antikoerper, l(1)G0229 antikoerper, l(1)G0278 antikoerper, l(1)top1 antikoerper, top1 antikoerper, topo I antikoerper, topoI antikoerper, DNA topoisomerase 1 beta antikoerper, DNA topoisomerase I alpha antikoerper, MGO1 antikoerper, MGOUN 1 antikoerper, MTE17.1 antikoerper, MTE17_1 antikoerper, TOP1BETA antikoerper, TOPOISOMERASE 1 antikoerper, TOPI antikoerper, AI467334 antikoerper, D130064I21Rik antikoerper, Top-1 antikoerper, Ab2-086 antikoerper, topi antikoerper, Topoisomerase 1 antikoerper, DNA topoisomerase I antikoerper, DNA topoisomerase I alpha antikoerper, DNA topoisomerase 1 antikoerper, topoisomerase (DNA) I antikoerper, DNA topoisomerase I, gene 1 L homeolog antikoerper, Top1 antikoerper, top1 antikoerper, topA antikoerper, ZMO1193 antikoerper, MRAD2831_RS63095 antikoerper, Lferr_0016 antikoerper, PC1_2015 antikoerper, Za10_0136 antikoerper, Gbro_0575 antikoerper, Dd586_2108 antikoerper, Kvar_3044 antikoerper, Alvin_2069 antikoerper, Dacet_0849 antikoerper, Mrub_1615 antikoerper, Arnit_2962 antikoerper, Ndas_4953 antikoerper, Trad_2377 antikoerper, Acear_1653 antikoerper, Mahau_0844 antikoerper, Mesop_4109 antikoerper, Thein_1586 antikoerper, Theth_0241 antikoerper, TOP1ALPHA antikoerper, top-1 antikoerper, TOP1 antikoerper, top1.1.L antikoerper

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: DNA topoisomerase 1, DNA topoisomerase I, TOP1

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Gen-ID: 7150

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance