Histone 3 Antikörper (H3K27me3)
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- Target Alle Histone 3 (H3) Antikörper anzeigen
- Histone 3 (H3)
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Bindungsspezifität
- H3K27me3
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Histone 3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)
- Spezifität
- Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide.
- Kreuzreaktivität
- Arabidopsis, Caenorhabditis elegans (C. elegans), Daphnia, Fruchtfliege (Drosophila melanogaster), Maize/Corn (Zea mays), Maus, Poplar (Populus), Red Algae (Chondrus), Tomate (Solanum lycopersicum)
- Kreuzreaktivität (Details)
- wide range
- Aufreinigung
- Peptide affinity purified
- Güteklasse
- ChIP-seq Grade
- Immunogen
- synthetic peptide
- Top Product
- Discover our top product H3 Primärantikörper
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- Applikationshinweise
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ChIP/ChIP-seq 1 - 2 μg:ChIP
ELISA 1:100 - 1:500
Dot Blot 1:5,000
WB 1:1,000
IF 1:200 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.1 μg/μL
- Buffer
- PBS, 0.05 % azide, 0.05 % ProClin300
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
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BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding." in: Protein & cell, (2022) (PubMed).
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BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding." in: Protein & cell, (2022) (PubMed).
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- Target
- Histone 3 (H3)
- Andere Bezeichnung
- Histone 3 (H3 Produkte)
- Synonyme
- H-3 antikoerper, histocompatibility 3 antikoerper, H3 antikoerper
- Hintergrund
- Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K27 is associated with inactive genomic regions.
- UniProt
- P68431
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