Rekombinanter SARS-CoV-2 Nucleocapsid Antikörper

Produktnummer ABIN6953059

Produktdetails anti-SARS-CoV-2 Nucleocapsid Antikörper

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antikörpertyp: Recombinant Antibody

Reaktivität: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV)

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser SARS-CoV-2 Nucleocapsid Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF)

Spezifität: This antibody binds the amino acid residues between 11-19 of the N protein of the SARS CoV as well as SARS-CoV-2 (COVID-19) nucleocapsid protein.

Produktmerkmale:

OriginalSpeciesName: Human

OriginalFormat: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatents were then purified on protein A columns. The original immunogen was the whole irradiated virion.

Klon: CR3018 (03-018)

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody is recommended for detection of SARS CoV2 protein N (nucleoprotein). This antibody binds both the nucleocapsid protein of the SARS-CoV and SARS CoV-2 (2019-nCoV). Initial characterization of the antibody for binding to 2019-nCoV was done using ELISA. This antibody shows potential to be used for development of diagnostic assays. Various isotype versions of the antibody namely human IgG1, IgG3, IgM, IgA and the less common IgG2 and IgG4 are available for the investigation of their role in response to SARS CoV2. Competitive ELISA of this antibody with CR3009 suggests that both these antibodies bind different epitopes of the N protein of SARS CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Immunofluorescence staining was used to demonstrate binding of CR3018 to SARS-CoV infected Vero cells.

Competitive ELISA of both anti-nucleocapsid antibodies suggests that they bind different, non-overlapping epitopes of the N protein of SARS-CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Clone CR3018 binds the amino acid residues between 11-19 of the N protein of SARS-CoV, while clone CR3009 binds a non-linear/conformational epitope of the N protein of SARS-CoV, both of which are sufficiently conserved to permit binding of these antibodies to SARS-CoV-2.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Referenzen für anti-SARS-CoV-2 Nucleocapsid Antikörper (ABIN6953059)

Gaynor, Vaysburd, Harman, Albecka, Jeffrey, Beswick, Papa, Chen, Mallery, McGuinness, Van Rietschoten, Stanway, Brear, Lulla, Ciazynska, Chang, Sharp, Neary, Box, Herriott, Kijak, Tatham, Bentley et al.: "Multivalent bicyclic peptides are an effective antiviral modality that can potently inhibit SARS-CoV-2. ..." in: Nature communications, Vol. 14, Issue 1, pp. 3583, (2023) (PubMed).

Ramm, Dondapati, Trinh, Wenzel, Walter, Zemella, Kubick: "The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins." in: Frontiers in bioengineering and biotechnology, Vol. 10, pp. 896751, (2022) (PubMed).

van den Brink, Ter Meulen, Cox, Jongeneelen, Thijsse, Throsby, Marissen, Rood, Bakker, Gelderblom, Martina, Osterhaus, Preiser, Doerr, de Kruif, Goudsmit: "Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus." in: Journal of virology, Vol. 79, Issue 3, pp. 1635-44, (2005) (PubMed).

Details zu SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Andere Bezeichnung: SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Produkte)

Synonyme: nucleocapsid phosphoprotein antikoerper, N antikoerper

Substanzklasse: Viral Protein

Hintergrund: NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV

UniProt: P0DTC9, P59595