Rekombinanter SARS-CoV-2 Nucleocapsid Antikörper
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- Target Alle SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) Antikörper anzeigen
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- Antikörpertyp
- Recombinant Antibody
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Reaktivität
- SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV)
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Wirt
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Human
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Klonalität
- Monoklonal
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Konjugat
- Dieser SARS-CoV-2 Nucleocapsid Antikörper ist unkonjugiert
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Applikation
- ELISA, Immunofluorescence (IF)
- Spezifität
- This antibody binds the amino acid residues between 11-19 of the N protein of the SARS CoV as well as SARS-CoV-2 (COVID-19) nucleocapsid protein.
- Produktmerkmale
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OriginalSpeciesName: Human
OriginalFormat: IgG1
- Aufreinigung
- Affinity Purified using a recombinant lectin column
- Immunogen
- The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatents were then purified on protein A columns. The original immunogen was the whole irradiated virion.
- Klon
- CR3018 (03-018)
- Isotyp
- IgA1 kappa
- Top Product
- Discover our top product SARS-CoV-2 N Primärantikörper
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- Applikationshinweise
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This antibody is recommended for detection of SARS CoV2 protein N (nucleoprotein). This antibody binds both the nucleocapsid protein of the SARS-CoV and SARS CoV-2 (2019-nCoV). Initial characterization of the antibody for binding to 2019-nCoV was done using ELISA. This antibody shows potential to be used for development of diagnostic assays. Various isotype versions of the antibody namely human IgG1, IgG3, IgM, IgA and the less common IgG2 and IgG4 are available for the investigation of their role in response to SARS CoV2. Competitive ELISA of this antibody with CR3009 suggests that both these antibodies bind different epitopes of the N protein of SARS CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Immunofluorescence staining was used to demonstrate binding of CR3018 to SARS-CoV infected Vero cells.
Competitive ELISA of both anti-nucleocapsid antibodies suggests that they bind different, non-overlapping epitopes of the N protein of SARS-CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Clone CR3018 binds the amino acid residues between 11-19 of the N protein of SARS-CoV, while clone CR3009 binds a non-linear/conformational epitope of the N protein of SARS-CoV, both of which are sufficiently conserved to permit binding of these antibodies to SARS-CoV-2. - Kommentare
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This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus." in: Journal of virology, Vol. 79, Issue 3, pp. 1635-44, (2005) (PubMed).
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Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus." in: Journal of virology, Vol. 79, Issue 3, pp. 1635-44, (2005) (PubMed).
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- Target
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- Andere Bezeichnung
- SARS-CoV-SARS-CoV-2 Nucleocapsid Protein2 N (SARS-CoV-2 N Produkte)
- Synonyme
- nucleocapsid phosphoprotein antikoerper, N antikoerper
- Substanzklasse
- Viral Protein
- Hintergrund
- NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV
- UniProt
- P0DTC9, P59595
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