Rekombinanter SARS-CoV-2 Spike S1 Antikörper

Produktnummer ABIN6953151

Produktdetails anti-SARS-CoV-2 Spike S1 Antikörper

Target: SARS-CoV-2 Spike S1

Antikörpertyp: Recombinant Antibody

Reaktivität: SARS Coronavirus-2 (SARS-CoV-2)

Wirt: Human

Klonalität: Chimeric

Konjugat: Dieser SARS-CoV-2 Spike S1 Antikörper ist unkonjugiert

Applikation: ELISA, Colloidal Gold Immunochromatography Assay (GICA)

Produktmerkmale: Recombinant anti-SARS-CoV-2 spike is expressed from 293 cells (HEK293) ,It is a chimeric monoclonal antibody combining the mouse variable regions with the constant domains of human IgG1.
Monoclonal mouse (varialbe region) / human (kappa / IgG1 constant)chimeric antibody.
AA 16-685

Aufreinigung: Affinity-chromatography

Immunogen: Recombinant Human Novel Coronavirus Spike glycoprotein (S) (16-685aa)

Klon: H6

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA 1:10000-1:50000
GICA 1:500-1:25000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 50 % Glycerol, 0.01M PBS, pH 7.4, 0.03 % Proclin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze

Details zu SARS-CoV-2 Spike S1

Target: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Produkte

Synonyme: E2 antikoerper, Surface Glycoprotein antikoerper, S antikoerper

Substanzklasse: Viral Protein

Hintergrund: Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.

Gen-ID: 43740568

UniProt: P0DTC2