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MHC II (I-A/I-E) Antikörper (violetFluor™ 450)

Reaktivität: Maus FACS Wirt: Ratte Monoclonal M5-114-15-2 violetFluor™ 450
Produktnummer ABIN7278570
  • Target Alle MHC II (I-A/I-E) Produkte
    MHC II (I-A/I-E)
    Reaktivität
    Maus
    Wirt
    • 33
    Ratte
    Klonalität
    • 33
    Monoklonal
    Konjugat
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MHC II (I-A/I-E) Antikörper ist konjugiert mit violetFluor™ 450
    Applikation
    • 31
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Aufreinigung
    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
    Klon
    M5-114-15-2
    Isotyp
    IgG2b kappa
  • Applikationshinweise
    This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.
    Kommentare

    0.2 mg/mL

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    2-8°C protected from light
    Haltbarkeit
    12 months
  • Target
    MHC II (I-A/I-E)
    Andere Bezeichnung
    MHC Class II (I-A/I-E) (MHC II (I-A/I-E) Produkte)
    Hintergrund
    The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.
    Gen-ID
    14961, 14969
    UniProt
    P14483
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