CD44 Antikörper (redFluor™ 710)

Produktnummer ABIN7278593

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch CD44 in FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für CD44 Antikörper (redFluor™ 710) (ABIN7278593)

Target: CD44

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD44 Antikörper ist konjugiert mit redFluor™ 710

Applikation: Flow Cytometry (FACS)

Klon: IM7

Produktdetails anti-CD44 Antikörper (redFluor™ 710)

Kreuzreaktivität: Pavian, Cat, Schimpanse, Cynomolgus, Hund, Pferd, Schwein, Rhesusaffen

Aufreinigung: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. redFluor™ 710 dye is excited by the red (633-647 nm) laser and has a peak emission of 710 nm. The recommended band pass filter for this dye is 710/50. redFluor™ 710 can be used as an alternative for Alexa Fluor® 700. Confirm that your cytometer is configured to detect this fluorochrome.

Kommentare:

0.2 mg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C protected from light

Haltbarkeit: 12 months

Details zu CD44

Target: CD44

Andere Bezeichnung: CD44

Hintergrund: The IM7 antibody recognizes CD44, a ubiquitously expressed cell surface receptor which is important for extracellular matrix organization, cell-cell and cell-matrix adhesion and migration. CD44 may be expressed in a number of different isoforms (splice variants) from the most typical or ""standard"" form, known as CD44s, to variants designated CD44v, e.g. CD44v1 or CD44v6. These receptors interact with several ligands, but most often associate with an extracellular matrix component hyaluronate, through which it mediates adhesion.The IM7 antibody may be used for detection of all isoforms of CD44, as it recognizes constant epitopes near the extracellular proximal domain. (Xu et al, 2002, J. Leukoc. Biol. 72:1133-1141). It has been reported to be cross-reactive with many non-human species including Baboon, Chimpanzee, Cynomolgus, Rhesus, Horse, Cow, Pig, Dog and Cat CD44.

Gen-ID: 12505, 960

UniProt: P16070, P15379

Pathways: Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling