IL-12/IL-23 p40 Antikörper

Produktnummer ABIN6964021

Produktdetails anti-IL-12/IL-23 p40 Antikörper

Target: IL-12/IL-23 p40

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-12/IL-23 p40 Antikörper ist unkonjugiert

Applikation: Neutralization (Neut)

Spezifität: Native and recombinant p40 subunit of human IL-12 and IL-23

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-23

Klon: MT3279H

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Neutralization of human IL-12 and IL-23 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: Neutralization of human IL-12 and human IL-23 induced IFN-γ production (see graphs). To determine the neutralizing capacity and ND50 on IL-12 induced IFN-γ production, mixtures of various concentrations of mAb MT3279H, human recombinant IL-12 (10 ng/mL) and recombinant IL-1β (50 ng/mL) were added to human PBMC. For determination of the neutralizing capacity and ND50 on IL-23 induced IFN-γ production, mixtures of various concentrations of mAb MT3279H, human recombinant IL-23 (10 ng/ ml) and recombinant IL-18 (50 ng/mL) were added to human PBMC. Following overnight incubation, cell supernatants were quantified in IFN-γ ELISA. Neutralization dose 50 (ND50): MT3279H neutralized the effect of IL-12 and IL-23 at a ND50value of 0.2-0.5 μg/mL when 10 ng/mL of recombinant human IL-12 or IL-23 was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS free of preservatives

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Referenzen für anti-IL-12/IL-23 p40 Antikörper (ABIN6964021)

Saghafian-Hedengren, Sohlberg, Theorell, Carvalho-Queiroz, Nagy, Persson, Nilsson, Bryceson, Sverremark-Ekström: "Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells." in: Journal of virology, Vol. 87, Issue 24, pp. 13446-55, (2014) (PubMed).

Details zu IL-12/IL-23 p40

Target: IL-12/IL-23 p40

Andere Bezeichnung: IL12/23 (p40) (IL-12/IL-23 p40 Produkte)

Gen-ID: 3592, 3593