5-Methylcytosine Antikörper

Produktnummer ABIN6971343

Produktdetails

Target: 5-Methylcytosine

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Methylated DNA Immunoprecipitation (MeDIP), Immunofluorescence (IF), Dot Blot (DB)

Produktmerkmale: 5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis. 5-Methylcytosine (5-mC) antibody (mAb) (Clone A1) was raised in a Mouse host. It has been validated for use in Dot blot, Immunofluorescence and Methyl DNA Immunoprecipitation, it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.

Aufreinigung: Protein A Chromatography

Immunogen: This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.

Klon: A1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Antigendetails

Target: 5-Methylcytosine

Andere Bezeichnung: 5-Methylcytosine (5-mC)

Substanzklasse: Chemical