Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)
Produktmerkmale
BORIS (Brother of Regulator of Imprinted Sites) / CTCFL (CTCF-like) is a zinc-finger DNA binding protein that acts as a chromatin insulator and a paralog of the CTCF protein. Normally, BORIS / CTCFL is expressed only in primary spermatocytes and is thought to play a role in resetting DNA methylation marks during the differentiation of male germ cells. BORIS / CTCFL is also expressed in many cancers and may serve a pro-proliferative function. Boris / CTCFL antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human samples.
Aufreinigung
Affinity Purified
Immunogen
This CTBP antibody was raised against a peptide within the N-terminal region of human BORIS.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.