Histone H2A Antikörper (ubLys119)

Produktnummer ABIN6971779

Produktdetails anti-Histone H2A Antikörper

Target: Histone H2A

Bindungsspezifität: ubLys119

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB)

Produktmerkmale: In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15 % of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies. Histone H2AK119ub antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a branched peptide corresponding to the ubiquitin conjugation site at Lys119 of human Histone H2A.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol, and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu Histone H2A

Target: Histone H2A

Abstract: Histone H2A Produkte

Synonyme: 5499 antikoerper, CG5499 antikoerper, Dmel\\CG5499 antikoerper, H2A antikoerper, H2A.F/Z antikoerper, H2A.X antikoerper, H2A.Z antikoerper, H2AV antikoerper, H2AV_DROM antikoerper, H2AX antikoerper, H2AZ antikoerper, H2Av antikoerper, H2AvD antikoerper, H2a.V antikoerper, H2av antikoerper, H2avD antikoerper, HIS antikoerper, HIS2AV antikoerper, HIS2AVD antikoerper, His antikoerper, His2 antikoerper, His2AV antikoerper, His2AvD antikoerper, HisH2Av antikoerper, Hist antikoerper, Hist2av antikoerper, gamma-H2Av antikoerper, gamma-HIS2AV antikoerper, gamma-His2Av antikoerper, gammaH2Av antikoerper, h2AvD antikoerper, his antikoerper, l(3)05146 antikoerper, l(3)810 antikoerper, l(3)97Dd antikoerper, l(3)L1602 antikoerper, DDBDRAFT_0205974 antikoerper, DDBDRAFT_0216271 antikoerper, DDB_0205974 antikoerper, DDB_0216271 antikoerper, RGD1564767 antikoerper, H2a-221 antikoerper, H2A-III antikoerper, HIST1H2AA antikoerper, Histone H2A variant antikoerper, putative histone H2A antikoerper, histone H2A antikoerper, histone cluster 1 H2A family member F antikoerper, histone H2A-like antikoerper, histone cluster 1, H2af antikoerper, histone2A1 antikoerper, histone cluster 1, H2A, III antikoerper, histone cluster 1, H2ac antikoerper, His2Av antikoerper, LMJF_17_0280 antikoerper, H2AX antikoerper, Hist1h2af antikoerper, LOC100425378 antikoerper, his2a1 antikoerper, HIST1H2A3 antikoerper, HIST1H2AC antikoerper, h2a-A antikoerper

Molekulargewicht: 23 kDa

NCBI Accession: NP_003508