Histone 3 Antikörper (acLys9)

Produktnummer ABIN6972014

Produktdetails anti-Histone 3 Antikörper

Target: Histone 3 (H3)

Bindungsspezifität: acLys9

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Produktmerkmale: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys9 can also be mono-, di- or trimethylated. The methylation of this residue is often associated with transcriptional repression. However, acetylation of histone H3 Lys9 is associated with transcriptional activation of the genes. Histone H3K9ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, CUT&Tag, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Protein A Chromatography

Immunogen: This Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of histone H3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended antibody dilutions for different applications:
ChIP: 10 µg per ChIP
ChIP-Seq: 3 µg each
ICC/IF: 2 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution
CUT&Tag: 1 µg per 50 µl reaction
Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Referenzen für anti-Histone 3 Antikörper (ABIN6972014)

Kee, Thudium, Renner, Glastad, Palozola, Zhang, Li, Lan, Cesare, Poleshko, Kiseleva, Truitt, Cardenas-Diaz, Zhang, Xie, Kotton, Alysandratos, Epstein, Shi et al.: "SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry." in: Nature, (2022) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3)

Andere Bezeichnung: Histone H3 (H3 Produkte)

Synonyme: H-3 antikoerper, histocompatibility 3 antikoerper, H3 antikoerper

Molekulargewicht: 17 kDa

NCBI Accession: NP_003522