Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Supershift Assay (SSA)
Produktmerkmale
MAZ (Myc-associated zinc finger protein) functions as a transcription factor roles in both transcription initiation and termination. MAZ binds to two sites within the c-Myc promoter, ME1a1 and ME1a2. It also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors. MAZ also participates in skeletal muscle development through the activation of several skeletal muscle-specific genes. MAZ has also been reported to drive tumor-specific expression of PPAR-gamma in breast cancer cells. MAZ antibody (mAb) (Clone 133) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Supershift and Western blot, it has been shown to react with Human samples.
Aufreinigung
Protein G Chromatography
Immunogen
This MAZ antibody was raised against recombinant human MAZ protein.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Target
MAZ
(MYC-Associated Zinc Finger Protein (Purine-Binding Transcription Factor) (MAZ))