TET1 Antikörper (C-Term)

Produktnummer ABIN6972860

Produktdetails anti-TET1 Antikörper

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TET1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Produktmerkmale: Tet1 (Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Tet1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunohistochemistry and Western blot, it has been shown to react with Human and Mouse samples.

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of mouse Tet1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu TET1

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Andere Bezeichnung: Tet1 (TET1 Produkte)

Synonyme: CXXC6 antikoerper, LCX antikoerper, bA119F7.1 antikoerper, 2510010B09Rik antikoerper, AA517754 antikoerper, BB001228 antikoerper, Cxxc6 antikoerper, D10Ertd17e antikoerper, mKIAA1676 antikoerper, tet methylcytosine dioxygenase 1 antikoerper, solute carrier family 25 member 16 antikoerper, TET1 antikoerper, Tet1 antikoerper, SLC25A16 antikoerper

Molekulargewicht: 215 kDa

NCBI Accession: NP_081660

Pathways: Stem Cell Maintenance, Warburg Effekt