Dieser SARS-Coronavirus Envelope Protein Antikörper ist unkonjugiert
Applikation
ELISA
Aufreinigung
SARS-CoV Envelope Antibody is affinity chromatography purified via peptide column.
Immunogen
Anti-SARS-CoV Envelope antibody was raised against a peptide corresponding to 11 amino acids near the carboxy terminus of SARS-CoV Envelope protein. The immunogen is located within the last 50 amino acids of SARS-CoV Envelope.
SARS-CoV Envelope antibody can be used for the detection of SARS-CoV Envelope protein in ELISA. It will detect 5 ng of free peptide at 1 μ,g/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
SARS-CoV Envelope Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
SARS-CoV Envelope antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
SARS Envelope Antibody: A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2. Envelope protein is a small polypeptide that contains at least one ?-helical transmembrane domain. It involves in several aspects of the virus's life cycle, such as assembly, budding, envelope formation, and pathogenesis. E protein has membrane permeabilizing activity, which provides a possible rationale to inhibit in vitro ion channel activity of some synthetic coronavirus E proteins, and also viral replication.