PHAP III Antikörper (C-Term)

Produktnummer ABIN6990425

Produktdetails anti-PHAP III Antikörper

Target: PHAP III

Bindungsspezifität: AA 200-250, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: PHAP III has no cross-reaction to PHAP I and PHAP I2a.

Aufreinigung: PHAP III Antibody is affinity chromatography purified via peptide column.

Immunogen: PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III. The immunogen is located within amino acids 200 - 250 of PHAP III.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: PHAP III antibody can be used for detection of PHAP III by Western blot at 1 μ,g/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human and rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PHAP III Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PHAP III antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu PHAP III

Target: PHAP III

Abstract: PHAP III Produkte

Hintergrund: PHAP III Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

Molekulargewicht: 35 kDa

Gen-ID: 81611

NCBI Accession: NP_112182

UniProt: Q9BTT0