Western Blotting (WB), ELISA, Immunofluorescence (IF)
Aufreinigung
TLR10 Antibody is Ion exchange chromatography purified.
Immunogen
TLR10 antibody was raised against a peptide corresponding to 14 amino acids near the middle of human TLR10. The immunogen is located within amino acids 600 - 650 of TLR10.
TLR10
Reaktivität: Human
FACS
Wirt: Maus
Monoclonal
3C10C5
unconjugated
Applikationshinweise
TLR10 antibody can be used for detection of TLR10 by Western blot at 0.5 to 1 μ,g/mL. Despite its predicted size of ~90 kDa, a band correpsonding to TLR10 is observed at ~75 kDa in immunoblot assays. For immunofluorescence start at 2 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
TLR10 Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
TLR10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
TLR10 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR10 is highly homologous to TLRs 1 and 6 and is most highly expressed in lymphoid tissues. Most recently genetic variation in TLR10 has been associated with contributing to asthma risk. It should be noted that TLR10 exists in mice only as a pseudogene.