ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Aufreinigung
West Nile Virus Core Antibody is affinity chromatography purified via peptide column.
Immunogen
West Nile Virus Core antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of the West Nile Virus core protein. The immunogen is located within amino acids 80 - 130 of West Nile Virus Core.
WNV Core
Reaktivität: West Nile Virus (WNV)
ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
West Nile virus Core antibody can be used for the detection of the WNV Core protein in ELISA. It will detect 10 ng of free peptide at 1 μ,g/mL. This antibody will also detect the protein in immunoblot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in monkey samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
West Nile Virus Core Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
West Nile Virus Core antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
WNV Core
(West Nile Virus Genome polyprotein (WNV Core))
West Nile Virus Core Antibody: West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.