ST8SIA4 Antikörper (N-Term)

Produktnummer ABIN6990643

Produktdetails anti-ST8SIA4 Antikörper

Target: ST8SIA4 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 4 (ST8SIA4))

Bindungsspezifität: AA 80-130, N-Term

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ST8SIA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Pst1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Pst1 antibody was raised against a synthetic peptide from the amino terminus of yeast Pst1. The immunogen is located within amino acids 80 - 130 of Pst1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Pst1 antibody can be used for detection of Pst1 by Western blot and ELISA at 1 μ,g/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Pst1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Pst1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu ST8SIA4

Target: ST8SIA4 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 4 (ST8SIA4))

Andere Bezeichnung: Pst1 (ST8SIA4 Produkte)

Synonyme: PST antikoerper, PST-1 antikoerper, SIAT8-D antikoerper, ST8SiaIV antikoerper, Siat8d antikoerper, PST1 antikoerper, SIAT8D antikoerper, ST8SIA-IV antikoerper, ST8Sia-IV antikoerper, pst antikoerper, siat 8D antikoerper, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 antikoerper, St8sia4 antikoerper, ST8SIA4 antikoerper, st8sia4 antikoerper

Hintergrund: Pst1 Antibody: Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein (Catalog No. 95-151) was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (Δ,och1 Δ,mnn1 Δ,mnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.

Molekulargewicht: 49 kDa

Gen-ID: 851625

UniProt: Q12355