Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
Spezifität
This antibody is predicted to not cross-react with the highly homologous Slc35D2.
Aufreinigung
Slc35D1 Antibody is affinity chromatography purified via peptide column.
Immunogen
Slc35D1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Slc35D1. The immunogen is located within the last 50 amino acids of Slc35D1.
Slc35D1 antibody can be used for detection of Slc35D1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Slc35D1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
Slc35D1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
SLC35D1
(Solute Carrier Family 35 (UDP-Glucuronic Acid/UDP-N-Acetylgalactosamine Dual Transporter), Member D1 (SLC35D1))
UGTREL7 antikoerper, AI834976 antikoerper, C330011J09 antikoerper, mKIAA0260 antikoerper, UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter antikoerper, solute carrier family 35 member D1 antikoerper, solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1 antikoerper, Tc00.1047053510611.20 antikoerper, Tc00.1047053506793.40 antikoerper, SLC35D1 antikoerper, Slc35d1 antikoerper
Hintergrund
Slc35D1 Antibody: The solute carrier family Slc35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The role of the ER-resident Slc family member Slc35D1 is to transport both UDP-glucuronic acid and UDP-N-acetylgalactosamine. These molecules can serve as substrates for chondroitin sulfate biosynthesis and mice lacking the Slc35D1 gene developed a lethal form of skeletal dysplasia with severe shortening of limbs and facial structures. Examination of epiphyseal cartilage in these mice revealed a decreased proliferating zone with round chrondrocytes, scarce matrices, and reduced proteoglycan aggregates. Loss of function mutations in human Slc35D1 cause Schneckenbecken dysplasia, a severe skeletal dysplasia.