ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Spezifität
At least two isoforms of MettL7A are known to exist. This antibody is predicted to not cross-react with MettL7B.
Aufreinigung
MettL7A Antibody is affinity chromatography purified via peptide column.
Immunogen
MettL7A antibody was raised against a 13 amino acid synthetic peptide near the center of human MettL7A. The immunogen is located within amino acids 130 - 180 of MettL7A.
METTL7A
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
Biotin
Applikationshinweise
MettL7A antibody can be used for detection of MettL7A by Western blot at 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
MettL7A Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
MettL7A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
MettL7A Antibody: MettL7A belongs to the methyltransferase superfamily. It is a probable methyltransferase. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Often methylation occurs on nucleic bases in DNA or amino acids in protein structures. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice they caused a recessive lethal phenotype. Methylation may also be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.