PIG-Y Antibody is affinity chromatography purified via peptide column.
Immunogen
PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y. The immunogen is located within amino acids 50 - 100 of PIG-Y.
PIG-Y antibody can be used for detection of PIG-Y by Western blot at 1 - 2 μ,g/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
PIG-Y Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
PIG-Y antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
PIGY
(Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))
PIG-Y antikoerper, pig-y antikoerper, Prey antikoerper, Pyurf antikoerper, prey antikoerper, 1810008A14Rik antikoerper, Pigy antikoerper, phosphatidylinositol glycan anchor biosynthesis class Y antikoerper, phosphatidylinositol glycan anchor biosynthesis, class Y antikoerper, Pigy upstream reading frame S homeolog antikoerper, phosphatidylinositol glycan anchor biosynthesis, class Y-like antikoerper, PIGY antikoerper, pigy antikoerper, Pigy antikoerper, pyurf.S antikoerper, Pigyl antikoerper
Hintergrund
PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.