Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
At least two isoforms of LRFN5 are known to exist. This antibody is predicted to not cross-react with other members of the LRFN family.
Aufreinigung
LRFN5 Antibody is affinity chromatography purified via peptide column.
Immunogen
LRFN5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human LRFN5. The immunogen is located within amino acids 650 - 700 of LRFN5.
LRFN5
Reaktivität: Säugetier
IHC, ISt
Wirt: Maus
Monoclonal
N140A-12
unconjugated
Applikationshinweise
LRFN5 antibody can be used for detection of LRFN5 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
LRFN5 Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
LRFN5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
LRFN5
(Leucine Rich Repeat and Fibronectin Type III Domain Containing 5 (LRFN5))
C14orf146 antikoerper, FIGLER8 antikoerper, SALM5 antikoerper, AI427653 antikoerper, AI604817 antikoerper, C130061B21 antikoerper, mKIAA4208 antikoerper, leucine rich repeat and fibronectin type III domain containing 5 antikoerper, LRFN5 antikoerper, Lrfn5 antikoerper
Hintergrund
LRFN5 Antibody: LRFN5 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while LRFN2 and LRFN5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system.