Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Spezifität
At least four isoforms of SDHD are known to exist, this antibody will detect the two longest isoforms.
Aufreinigung
SDHD Antibody is affinity chromatography purified via peptide column.
Immunogen
SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD. The immunogen is located within amino acids 30 - 80 of SDHD.
SDHD
Reaktivität: Human, Ratte
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
AbBy Fluor® 594
Applikationshinweise
SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
SDHD Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
SDHD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
SDHD
(Succinate Dehydrogenase Complex, Subunit D, Integral Membrane Protein (SDHD))
SDHD Antibody: The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.