ELISA, Western Blotting (WB), Immunofluorescence (IF)
Spezifität
At least five alternatively spliced transcript variants have been observed. COH1 detects two isoforms.
Aufreinigung
COH1 Antibody is affinity chromatography purified via peptide column.
Immunogen
COH1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human COH1. The immunogen is located within amino acids 90 - 140 of COH1.
COH1 antibody can be used for detection of COH1 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
COH1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
COH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
VPS13B
(Vacuolar Protein Sorting 13 Homolog B (Yeast) (VPS13B))
COH1 Antibody: COH1 (Cohen syndrome protein 1), also known as VPS13B (vacuolar protein sorting-associated protein 13B) or CHS1, belongs to the VPS13 family and may function in vesicle-mediated transport and sorting of proteins within the cell. COH1 is widely expressed and multiple alternatively spliced transcript variants have been observed. Mutations in this gene have been associated with Cohen syndrome. COH1 is a Golgi-localized peripheral membrane protein and plays a critical role in Golgi (re)assembly.