LIMA1 Antikörper (N-Term)

Produktnummer ABIN6992089

Produktdetails anti-LIMA1 Antikörper

Target: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Bindungsspezifität: AA 230-280, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIMA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist, this antibody will only detect the three largest isoforms.

Aufreinigung: LIMA1 antibody is affinity chromatography purified via peptide column.

Immunogen: LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1. The immunogen is located within amino acids 230 - 280 of LIMA1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: LIMA1 antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: LIMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu LIMA1

Target: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Andere Bezeichnung: LIMA1 (LIMA1 Produkte)

Hintergrund: The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).

Molekulargewicht: Predicted: 50, 66, 84 kDa

Observed: 65 kDa

Gen-ID: 51474

NCBI Accession: NP_001107018

UniProt: Q9UHB6