Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
Spezifität
RSPO3 antibody is human and mouse reactive. At least three isoforms of RSPO3 are known to exist, this antibody will detect all three.
Aufreinigung
RSPO3 antibody is affinity chromatography purified via peptide column.
Immunogen
RSPO3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RSPO3. The immunogen is located within amino acids 200 - 250 of RSPO3.
RSPO3
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
Biotin
Applikationshinweise
RSPO3 antibody can be used for detection of RSPO3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
RSPO3 antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
RSPO3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
RSPO3 is a member of the R-spondin family and plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis (1,2). RSPO3 has been shown to be involved in regulating the balance between angioblast and blood cell specification during embryonic vasculogenesis and angiogenesis and may be involved in tumor development (3,4).