Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Spezifität
RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist, this antibody will detect both isoforms.
Aufreinigung
RFX1 antibody is affinity chromatography purified via peptide column.
Immunogen
RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1. The immunogen is located within the last 50 amino acids of RFX1.
RFX1
Reaktivität: Human
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
RFX1 antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
RFX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
RFX1
(Regulatory Factor X, 1 (Influences HLA Class II Expression) (RFX1))
EFC antikoerper, RFX antikoerper, AI047719 antikoerper, AI385641 antikoerper, zgc:162914 antikoerper, regulatory factor X1 antikoerper, regulatory factor X, 1 (influences HLA class II expression) antikoerper, regulatory factor X, 1a (influences HLA class II expression) antikoerper, RFX1 antikoerper, Rfx1 antikoerper, rfx1a antikoerper
Hintergrund
RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1,2). RFX1 is structurally related to regulatory factors X2, X3, X4, and X5 (3). RFX1 is a regulatory factor essential for MHC class II genes expression and can bind to an inverted repeat that is required for expression of hepatitis B virus genes (4). Recent study identifies RFX1 may play a tumor suppressor role in HCC as autophagy mediator (5).