ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist, this antibody will detect the two longest isoforms.
Aufreinigung
IZUMO1 antibody is affinity chromatography purified via peptide column.
Immunogen
IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1. The immunogen is located within amino acids 170 - 220 of IZUMO1.
IZUMO1
Reaktivität: Human
ELISA, WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
IZUMO1 antibody can be used for detection of IZUMO1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
IZUMO1 antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
IZUMO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3). Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4).