ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
JAGN1 antibody is human, mouse and rat reactive. At least four isoforms of JAGN1 are known to exist, this antibody will detect all but isoform 2.
Aufreinigung
JAGN1 antibody is affinity chromatography purified via peptide column.
Immunogen
JAGN1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAGN1. The immunogen is located within the last 50 amino acids of JAGN1.
JAGN1
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
JAGN1 antibody can be used for detection of JAGN1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
JAGN1 antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
JAGN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
JAGN1 is the human ortholog of a gene originally identified in Drosophila melanogaster. It is a transmembrane protein that functions in the early secretory pathway and is necessary for neutrophil differentiation and survival. Individuals with mutations in the JAGN1 gene causes aberrant myeloid cell homeostasis and severe congenital neutropenia (SCN) (1). JAGN1-mutant granulocytes are characterized by ultrastructural defects, aberrant N-glycosylation of multiple proteins, and increased apoptosis (1). Mice lacking this gene could not mount and efficient neutrophil-dependent immune response to a human fungal pathogen (2).