Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
Spezifität
IL-11 antibody is human specific. At least two isoforms of IL-11 are known to exist, this antibody will only detect the longest isoform of IL-11.
Aufreinigung
IL-11 Antibody is affinity chromatography purified via peptide column.
Immunogen
IL-11 antibody was raised against a 16 amino acid peptide from near the amino terminus of human IL-11. The immunogen is located within amino acids 40 - 90 of IL-11.
IL-11 antibody can be used for the detection of IL-11 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry at 10 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
IL-11 Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
IL-11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Interleukin 11 is a pleiotropic cytokine that has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6 (1). It directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (2). IL-11 promotes the proliferation of hepatocytes in response to liver damage. IL-11 binds to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 (3). Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelo-suppressive chemotherapy (4).